The lab says fixation will result in the dyes to leak from the cells. I believe the belief is fixation may possibly damage cell membranes. I did locate a paper which describes fixation making use of methanol or acetic acid, right after staining with a DCFDA spinoff. DCFDA could be https://claytonixiuf.weblogco.com/28083883/the-2-minute-rule-for-2-fdck-hcl